earl’s buffered salt solution (ebss) (Thermo Fisher)

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Thermo Fisher earl’s buffered salt solution (ebss)

Earl’s Buffered Salt Solution (Ebss), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+ebss/chemicals+and+buffers/pmc11578587-227-6-10
Average 90 stars, based on 1 article reviews

earl’s buffered salt solution (ebss) - by Bioz Stars, 2026-11

90/100 stars

Images

1) Product Images from "Motor neurons are dispensable for the assembly of a sensorimotor circuit for gaze stabilization"

Article Title: Motor neurons are dispensable for the assembly of a sensorimotor circuit for gaze stabilization

Journal: eLife

doi: 10.7554/eLife.96893

Figure Legend Snippet:

Techniques Used: Electron Microscopy, Recombinant, In Situ Hybridization, Isolation, Purification, Sequencing, Software, CRISPR, Flow Cytometry

Modification:

Article Title: Loss of Lamp2a-dependent chaperone-mediated autophagy drives dry AMD-like retinal pathology in mice and is rescued by BK channel activation
Article Snippet: Polybrene (107689), 4′,6-diamidino-2-phenylindole (DAPI, D1306) and Bafilomycin A1 (SML1661) were purchased from Sigma. .. Dulbecco’s modified Eagle medium (DMEM, 10313021), fetal bovine serum (FBS), Ham’s F-12 nutrient mix (11765054), Earle’s balanced salt solution (EBSS, 14-155-063), Dulbecco’s phosphate buffered saline (DPBS), Lipofectamine 300 transfection reagent (L3000015), polyvinylidenedifluoride (PVDF, LC2002) membrane and protease inhibitor cocktail were obtained from Thermo Scientific. .. Antibodies against LC3B (Sigma, L8918-200UL), actin (Proteintech, 66009-1-Ig), PLIN2 (Proteintech, 15294-1-AP), fibronectin (Invitrogen, PA5-29578), clusterin (R&D systems, AF2747), vitronectin (R&D Systems, MAB38751), and p62 (Abnova, H00008878-M01; CST, 5114), Lamp2a (Abcam, ab125068), Lamp2b (Abcam, ab13524), Iba1 (Fujifilm Wako, 019-19741), Laminin (Sigma, L9393) and ApoE (Abcam, ab183596) were used in this study.

Saline:

Transfection:

Membrane:

Protease Inhibitor:

other:

Article Title: Super-resolution morphology-correlated detection of labile zinc
Article Snippet: The 1H NMR and 13C NMR spectra were determined with a 400 M Bruker spectrometer with TMS as internal standard.

Isolation:

Article Title: TET2-dependent differential 5hmC deposition balances adult neural stem cell activation and differentiation
Article Snippet: .. To initiate each independent culture, the brains of two different animals were dissected out and the regions containing the V-SVZ from each hemisphere were isolated and washed in Earle’s balanced salt solution (EBSS; Gibco). .. Tissues were transferred to EBSS containing 12 U/mL papain (Worthington DBA), 0.2 mg/ ml L-cystein (Sigma-Aldrich), 0.2mg/mL EDTA (Sigma-Aldrich), and incubated for 20 min at 37 °C.

Article Title: TET3 protects the Dlk1-Dio3 imprinted locus from DNA hypomethylation during adult NSC reprogramming
Article Snippet: .. To initiate each independent culture, the brains of two different animals were dissected and the regions containing the SVZ were isolated from each hemisphere and washed in Earle’s balanced salt solution (EBSS; Gibco). .. Tissues were transferred to EBSS containing 12 U mL -1 papain (Worthington), 0.2 mg mL -1 L-cystein (Sigma-Aldrich), 0.2 mg mL -1 EDTA (Sigma-Aldrich) and incubated for 20 min at 37°C.

Lysis:

Article Title: Anticancer efficacy of 2-Hydroxy-4-methoxybenzoic acid through dual regulation of lactate transport via inhibition of MCT-1 and MCT-4
Article Snippet: .. HMBA (578-39-2) and Syro (SU-3118) were purchased from Sigma Aldrich; DMEM (Thermo Fischer; 11966065); Dextrose (Finer; CASR-5099-7); Foetal Bovine Serum (FBS; Gibco; 10270); 4′,6-diamidino-2phenylindole (DAPI; Sigma Aldrich; D9542); Eagle balanced salt solution (EBSS; Gibco, -2018-11); Hank’s balanced salt solution (HBSS; Himedia; TL1190); 7,12- dimethylbenz[a]anthracene (DMBA; Sigma Aldrich; 57-97-6); Collagenase type 4 (Himedia; TC-214); hyaluronidase (Himedia; TC331); RIPA lysis buffer (Amresco; N653); hematoxylin (Himedia; S058); sodium cacodylate (Sigma Aldrich; C0250); eosin (Himedia; S007); bovine serum albumin (BSA; Himedia; TC548); protein assay kit (Amresco; M173); 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT; TC1991-1G); Acridine orange (AO; Himedia; MB116); Ethidium bromide(EtBr; Himedia; MB071); 5,5,6,6′- tetrachloro- 1,1′,3,3′-tetraethylbenzimi-dazoylcarbocyanine iodide (JC1; Cayman chemical; 100009172). .. All other chemicals were of molecular biology grade and procured from Genetix Biotech Asia Pvt.

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